Microwave-assisted digestion as a novel technique for sample pretreatment has been paid more attention to.
微波消解作为样品预处理的新技术已受到普遍关注。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
材料和方法:采用酶辅助的显微分离法分离新生牛眼ipe细胞,酶消化法分离角膜内皮细胞,进行体外细胞培养。
Methods IPE and RPE cells were isolated by enzymatic digestion method and enzyme-assisted microdissection method respectively.
方法采用酶消化法及酶辅助机械分离法分别分离IPE和RPE细胞。
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