Methods: An alkaline lysis method was used to isolate serum HBV DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清HBVDNA,比较荧光定量PCR测定的重复性和测定值的差异。
参考来源 - 血清乙肝病毒DNA的快速提取及定量PCR测定中应用的探讨·2,447,543篇论文数据，部分数据来源于NoteExpress
Plasmid DNA was extracted by alkaline lysis method.
碱裂解法提取质粒。
The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn.
采用碱裂解法与DEAE -纤维柱层析法提取与纯化抑制素质粒dna，用分光光度法测定其浓度和纯度。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
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