Methods: An alkaline lysis method was used to isolate serum HBV DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清HBVDNA,比较荧光定量PCR测定的重复性和测定值的差异。
参考来源 - 血清乙肝病毒DNA的快速提取及定量PCR测定中应用的探讨·2,447,543篇论文数据，部分数据来源于NoteExpress
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