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Methods The total indole alkaloid was extracted after the calli were treated with fungal elicitors. Then, the determination of ajmalicine and catharanthine was carried out by RP HPLC.
方法用真菌诱导子对长春花愈伤组织进行诱导处理后提取吲哚总碱,并用RP- HPL C法测定其中阿玛碱和长春质碱含量。
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