Conclusion:Thiocyanate elution is a powerful and effective method to help obtain the Phabs with high affinity from phage antibody library.

结论：硫氰酸盐洗脱法用于噬菌体抗体的筛选有助于成功获得期望的高亲和力抗体。

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Objective To establish a method for the measurement of relative affinity of phage antibodies. Methods The relative affinities of 5 phage antibodies were measured by ELISA using thiocyanate elution.

亲和力是反映抗体特性的一个重要指标，也是抗体研究中经常需要测定的参数，在抗体的筛选、鉴定乃至其功能和应用研究等方面都涉及到亲和力的问题。

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