3 Established the IL-2 gene recombinant adenovirus vector.
3、人IL-2基因腺病毒载体的构建和包装。
参考来源 - ILObjective:To transfect the Dendritic cells (DC) with recombinant adenovirus vector AdmIL-12,make vaccine of DC.
目的:应用重组腺病毒AdmIL-12转染树突状细胞(DC)制备DC疫苗。
参考来源 - 重组腺病毒AdmILFirst we used the adenovirus vector containing EGFP gene(rAd5-EGFP) to infect BHK21c022 cells at different MOI(from 0 to 1 000),and then evaluated their transduction efficiency and cytotoxicity.
首先用携带EGFP基因的腺病毒载体rAd5-EGFP以不同的腺病毒用量(MOI)(01 000)感染BHK21c022细胞,观察比较其转导效率和细胞毒性。
参考来源 - 腺病毒载体介导的重组蛋白sTNFRII·2,447,543篇论文数据,部分数据来源于NoteExpress
Established the IL-2 gene recombinant adenovirus vector.
人IL-2基因腺病毒载体的构建和包装。
Adenovirus vector is an earlier and maturer genetic carrier in the respect of gene therapy and genetic immunization after retroviral vector.
腺病毒载体是继逆转录病毒载体后在基因治疗、基因免疫等方面应用开发得较早且较成熟的一种基因载体。
Results Fiber-mutant adenovirus vector showed a notably enhanced gene expression in four different tumor cells compared with that of conventional ones.
结果:与野生型腺病毒相比,重组腺病毒能显著地提高目的基因在四种不同肿瘤细胞中的表达。
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