方法制作犬乳恒牙替换期牙槽骨组织切片，采用免疫组化检测破骨细胞中CNP的表达情况。
Methods By immunohistochemical experiment on different period of dog's alveolar bones to find if CNP were expressed in osteoclasts.
结论低温塑料包埋方法制备的人类不脱钙骨组织切片可同时进行免疫组化和原位杂交的检测观察。
Conclusions The undecalcified bone sections of human made in the method can satisfy the need of IH and ISH.
方法：采用改进的甲基丙稀酸甲酯低温塑料包埋法包埋SD大鼠胫骨近端，制备不脱钙的骨组织切片。
Methods The proximal tibiae of SD rats were embedded with the modified methylmethacrylate low-temperature embedding method and then made into undecalcified bone sections.
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