Influences of different cryopreservation protocols and preculture mediums on cells regrowth rate were significant.
5.不同的预培养方式和冷冻方法均对冻存后细胞的再生长影响很大。
参考来源 - 日本结缕草（Zoysia japonica Steud.）悬浮再生体系的建立与超低温保存研究The factors influencing the transformation efficiency for agrobacterium-mediated methods were mainly studied the pre-culture, the concentration of agrobacterium, the time of infection and the effect of AS.
3．影响遗传转化的因素主要探讨了预培养、菌液浓度和侵染时间、AS。
参考来源 - 东北玉米自交系Bt基因遗传转化的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
短期高温预培养可明显提高愈伤组织的产量；
The yield of callus increased apparently by higher temperature preculture for a short time.
结果表明：经预培养的转化愈伤组织中的荧光表达量明显高于未经预培养的转化子；
The results showed that the fluorescence expression of pre-cultured transformed callus was significantly higher than the transformed callus without pre-culture after the infection of Agrobacterium.
研究了微束激光转化法用于棉花感受态萌动胚转化预培养的时间、高渗液对材料的处理等。
The recent advances about laser microbeam in plant transformation of exogenous gene, key techniques and applications are introduced.
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