非洲猪瘟病毒（ASFV, African swine fever virus）是非洲猪瘟病毒科（Asfarviridae）下仅有的种，具有传染性和极高的致病性。急性病例临床症状以高热、病程短、死亡率高、内脏器官广泛性出血以及呼吸系统和神经系统功能紊乱为主要特征。猪瘟病毒的3D精细结构已经被破译，但直至2020年初仍未有针对ASFV的特效疫苗或是抗病毒药物可以有效的在疫情爆发时及时控制病毒的传播。
本发明公开了一种用于检测非洲猪瘟病毒抗体的竞争ELISA试剂盒及其用途，属于生物技术领域。
The invention discloses a competitive ELISA kit for detecting african swine fever virus antibody and the purposes thereof, which belong to the biological technical field.
试剂盒采用原核表达的重组P 30蛋白作为包被抗原，依据竞争ELISA原理检测猪血清中非洲猪瘟病毒的抗体。
The kit adopts prokaryotic expression recombinant P30 protein as coating antigen, and detects the African swine fever virus antibody in pig serum according to a competitive ELISA principle.
“非洲猪瘟病毒向高加索地区的蔓延给家畜健康带来极大的威胁并有可能导致出现严重局面，”粮农组织首席兽医官约瑟夫·多梅内奇说。
"The spread of the African Swine Fever virus to the Caucasus region poses a very serious animal health risk and could lead to a dramatic situation," said FAO Chief Veterinary Officer Joseph Domenech.
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