名词定义中文名称：随机聚合酶链反应英文名称：random PCR其他名称：随机PCR定义：采用许多序列不相同的(部分随机或全部随机序列)、短的(约10个核苷酸)引物所进行的聚合酶链反应。由于在模板DNA多个不同位置上扩增，所
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随机引物聚合酶链反应 arbitrarily primed polymerase chain reaction ; pcr with arbitrary primers ; AP-PCR
目的筛选出最佳随机引物并优化其反应体系用于8种细菌的随机引物聚合酶链反应（AP PCR）分析。
OBJECTIVE To screen the primers and optimize the reaction systems for arbitrarily primed polymerase chain reaction(AP-PCR) on 8 sorts of bacteria.
方法对医院临床分离的8株耐亚胺培南铜绿假单胞菌进行随机引物聚合酶链反应（ PCR）扩增，扩增产物进行电泳和聚类分析。
METHODS The genes of imipenem-resistant P. aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis.
方法 对116例随机收集的1型糖尿病患者用聚合酶链反应-限制性内切酶消化作该点突变的筛选；
Methods The mutation had been screened from 116 unrelated patients with type 1 diabetes mellitus by ApaI digestion of product of polymerase chain reaction amplification.
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