PCR产物经限制性内切酶消化、序列测定及分析。
方法：采用PCR SSCP法检测血管网织细胞瘤中VHL基因的突变率及甲基化，敏感限制性内切酶消化法检测血管网织细胞瘤中VHL基因的异常甲基化率。
Methods: The hypermethylation was examined by methyl sensitive restrictive DNA endoenzyme analysis in 34 cases of angioreticuloma and the VHL gene mutations detected by PCR SSCP analysis.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
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