小鼠骨髓内皮细胞经过长期培养后,已转化为内皮细胞株,已传代20次,其增殖能力没有减退。
Murine bone marrow endothelial cell line could be established after long time culture and there is no significant reduction in their proliferation potency after 20 passages.
方法:选取10例初诊或复发急性白血病患者的骨髓进行原代细胞培养,取对数生长期细胞接种于6孔板中;
Methods:Selected 10 patients with newly diagnosed or relapsed acute leukemia in patients with primary bone marrow cells were cultured in logarithmic growth phase cells were seeded in 6 well plate.
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