方法采用链霉蛋白酶消化、间断密度梯度离心纯化大鼠胃黏膜壁细胞。
Methods Rat gastric parietal cells were isolated by pronase digestion and purified by discontinuous density-gradient centrifugation.
经链霉蛋白酶E酶解获得的糖肽显著抑制精卵结合，提示BSL糖肽亦参与精卵结合。
The sperm-oocyte binding was markedly inhibited by the glycopeptide from BSL degradated by pronase E, suggesting that the glycopeptide of BSL may also take part in sperm-oocyte binding.
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