有目的基因，有质粒，然后用酶使两者相接就是重组质粒，重组质粒是一个种动作，方法，也是一个名词。
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...疡病菌致病基因pthAC-末端的3个核定位信号序列,并将其克隆到原核表达载体PET32a(+)上,经双酶切及核酸序列测定重组质粒(pthA-NLS),其序 ..
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用简单异尖线虫的 重组质粒 ( AS-ITS-pGM )为模板建立SYBR Green I荧光定量PCR检测简单异尖线虫的方法,并用该方法对经PCR-RFLP鉴定为简单异尖线虫的样品进行鉴定。
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recombinant plasmid - 引用次数：250
Recombinant plasmid was successfully constructed.
2重组质粒构建成功。
参考来源 - 构建稳定表达炎症因子TNFα、ILrecombination plasmid - 引用次数：6Nucleic acid vaccine is a recombination plasmid containing the code region of antigen gene.
核酸疫苗是由抗原编码基因和真核表达载体构建的重组质粒,将其导入机体后可诱导机体全面的免疫应答。
参考来源 - Sry核酸疫苗作用途径初探recombined plasmid - 引用次数：6Standard curves were also plotted using the 10-fold dilution of recombined plasmid.
将重组质粒系列十倍比稀释后绘制标准曲线。
参考来源 - 实时定量RTreorganization material particle - 引用次数：4Results:（1）The PCR product of reorganization material particle pcDNA3.1（-）-peb1A was the sole banding,and the size was 812bp by the agarose gel electrophoresis;the purity was 1.929 and the density was 2064μg/ml after using Qiagen plasmid Giga the Kit massive extraction.
结果:(1)重组质粒pcDNA3.1(-)-peb1A PCR扩增产物经琼脂糖凝胶电泳,为单一条带,大小为812bp;应用Qiagen plasmid Giga Kit大量抽提的纯度为1.929,浓度为2064μg/ml。
参考来源 - 空肠弯曲菌pcDNA3.1（ -
recombinant plasmid - 引用次数：87
The pcDNA-N recombinant plasmid was constructed and purified with PEG8000. With the method of calcium phosphate , the pcDNA-N was transfected into the COS-7 cells.
将pcDNA3-N真核表达重组质粒通过磷酸钙的方法转染COS-7细胞。
参考来源 - 猪繁殖与呼吸综合征病毒核衣壳蛋白基因在原核和真核系统中的表达与鉴定pcdnakcpg-1 - 引用次数：1 参考来源 - 对鸡具有免疫刺激活性的重组质粒pcDNAKCpG -
recombinant vector - 引用次数：7
The result showed that from the third day cell grow into logarithmic growth phase and on the eighth day grow into stagnate phase. The osteoblasts in logarithmic growth phase would be transfected by bFGF recombinant vector.
取对数生长期细胞进行bFGF重组质粒转染。
参考来源 - bFGF基因转染成骨细胞的生物学性质及成骨研究recombined plasmid - 引用次数：6Methods A signal peptide sequence(sp) was synthesized and cloned into the integrative plasmid pIlac down-stream of lactose-inducible promoter. The recombined plasmid pIlac-sp-IL10 was then introduced into Lactobacillus casei(L. casei)CECT 5276 by electroporation.
方法人工合成信号肽系列后与IL-10基因连接后克隆到干酪乳酸杆菌(Lactobacillus casei,L.casei)整合型表达载体pIlac的lac启动子下游,得到重组质粒pIlac-sp-IL10,电穿孔转化L.caseiCECT 5276。
参考来源 - 构建表达ILrecombination plasmid - 引用次数：3In this thesis the LBSP identification medium screening techniques was used. Only after one cycle, large quantities no-function recombination plamid and enzyme activity increasing 20 times recombination plasmid were obtained.
本研究用LBSP鉴别培养基直接筛选技术，仅一个循环就获得了大量无酶活的重组质粒和酶活提高了20倍的突变基因。
参考来源 - αrecombinant plasmid - 引用次数：1 参考来源 - 旋毛虫Ts87基因重组质粒的构建及在体外真核细胞中的表达
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双语例句原声例句
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重组质粒表达产物定位于细胞核；
Expression products of recombinant plasmid mainly located in nucleus.
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用化学法把重组质粒转化到E 。
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用酶切和PCR的方法对重组质粒进行鉴定。
Used restrict enzyme and PCR to verify the reconstructed plasmid.
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One way that you can look for that gene that you want is by making the cut in your plasmid inside of a gene that encodes for some property like resistance to an antibiotic.
一种找到成功重组质粒的方式,是在质粒控制某种特性的编码区域切割,例如,对抗生素的抗性
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Not every plasmid in your test tube is going to have the right gene inserted in the right way.
不是试管里的每个质粒,都会与正确的DNA片段重组
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Some are going to reform and some are going to reform with the gene in, and some probably aren't going to reseal at all under the conditions that I've used.
有些质粒会自己合上,有些质粒会与DNA片段重组并合上,还有的在我提供的条件下,也根本合不上
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