蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。
The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years.
实现其在CHO - DHFR -细胞中的高效表达，获取生物活性较高的重组蛋白。
AIM: TO obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR-cells.
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