经同源重组后,以终点稀释和斑点杂交法筛选重组杆状病毒;
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
目的探讨重组杆状病毒作为哺乳动物基因转导载体的可行性及其转导特点。
Objective to study the feasibility and the characteristics of recombinant baculovirus as mammalian gene transfer vector.
然后使用重组杆状病毒技术使这些S1亚单位在昆虫细胞和昆虫幼虫中实现了高水平表达。
The modified S1 subunits were abundantly expressed by recombinant autographa californica nuclear polyhedrosis viruses in insect cells and larva.
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