重叠延伸PCR overlap extension PCR
重叠延伸拼接 Overlapping PCR ; splicing by overlap extension ; splicing overlap extensive ; splicing by overlapping extension
重叠延伸拼接法 splicing by overlap extension ; splicing by overlapping extension ; SOE
应用重叠延伸剪切技术 splicing by overlapping extension
重叠延伸剪接技术 splice by overlapping extension ; splicing by overlap extension
引物重叠延伸法 splicing by overlap extension
采用重叠延伸剪接技术 splice by overlapping extension ; splicing by overlap extension
采用剪接式重叠延伸 spliced overlap extension ; SOE
用重叠延伸剪接技术 SOE ; splice by overlapping extension ; splicing by overlap extension
结论：重叠延伸pcr是一种获得重构基因的简捷方法。
Conclusion Splicing overlap extension by PCR is a convenient method to obtain recombinant gene.
重叠延伸PCR技术成功的关键是重叠互补引物的设计。
The overlap primers design is the key factor for the successful accomplishment of SOE PCR.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
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