从细胞萃取液或生物液体中分离酶的方法,基本上就是纯化蛋白质的方法。
The methods employed fOR enzyme isolation from cell extracts OR from biological fluids are essentially those fOR the purification of proteins.
本文的研究目的是筛选对2-氯丙酸具有脱氯能力的菌株,并建立脱卤酶的分离纯化方法,从而为利用脱卤酶生产(S)-及(R)-构型光学活性2-氯丙酸奠定基础。
This dissertation aimed to screen the strains and develop a purification method of dehalogenase. These will contribute to the product of optically activated (S)-2-CPA and (R)-2-CPA.
方法:将从小鼠肌肉分离、纯化的旋毛虫幼虫,放入玻璃匀浆器匀浆,胰酶消化。台盼蓝和中性红染色检测分离细胞的活性。
Methods: Larvae of Trichinella spiralis isolated from the muscle of a Trichinella - infected mouse were put in a glass homogenizer for homogenizing, digesting with trypsin.
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