提供的人血小板衍生生长因子-BB,酵母表达(PDGF BB Yeast)产品为经0.2μm孔径过滤灭菌的白色冻干粉末;该冻干粉末是从含人血小板衍生生长因子-BB,酵母表达(PDGF BB Yeast)的20mM 乙酸钠(...
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...:提供的人血管内皮生长因子,酵母表达(VEGF Yeast)产品为过滤灭菌的无色溶液形式;其中人血管内皮生长因子,酵母表达(VEGF Yeast)产品溶解于 20mM磷酸盐缓冲盐水(pH 7.4)溶液中。
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BACKGROUND AND AIM:To express the human fascin protein,an important tumor molecular marker,in Pichia pastoris. MATERIALS AND METHODS:The recombinant plasmid pPIC9-fascin was constructed by inserting the full coding DNA sequence of fascin into the secreted expression yeast vector pPIC9.
背景与目的:应用酵母表达系统表达人的重要肿瘤分子标志物fascin蛋白。
参考来源 - 应用毕赤酵母系统表达人Fascin蛋白The correctly modified HVl gene was inserted into yeast expression vector pYC-DE-2 and identified.
序列正确的HV1基因与酵母α因子信号肽基因相连,一起插入酵母表达载体pYC-DE-2。
参考来源 - 水蛭素基因的合成及在酵母中表达与产物纯化的研究But, commercial vaccine produced in yeast expression system is expensive for its higher production costs, and needs refrigeration. This made the universal vaccination difficult in remote areas and economically underdeveloped regions.
由于现在使用商业化HBV疫苗主要通过酵母表达系统生产,存在较高的生产成本,而且需要冷藏,因此对边远和经济不发达地区人群疫苗接种普及带来困难。
参考来源 - 乙型肝炎新型植物口服疫苗的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
Express ancrod, a snake venom thrombin-like enzyme, in methylotrophic yeast Pichia pastoris.
该突变体可在酵母表达系统实现高效表达。
The mutant may be he efficiency expressed in yeast expression system.
毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。
The Pichia pastoris is one of the most successful foreign protein expression system until now.
Took it out of the virus completely, cloned it into a plasmid, that plasmid was expressed in a foreign host, in this case it was expressed in yeast cells.
将基因从病毒中完全提取出来,并克隆制成质粒,该质粒在外来宿主中表达,此例中质粒在酵母细胞中表达
Yeast cells were grown in large numbers with this plasmid inside, they expressed the plasmid and so you made Hepatitis B surface antigen not in people but in cell culture where it was not normally formed.
体内携带这种质粒的酵母细胞大量增殖,它们表达质粒,因此,乙型肝炎表面抗原不是在人体内,而是在细胞培养环境下,以非正常方式形成的
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