目的研究胃泌素对人结肠癌细胞信号分子黏着斑激酶(FAK)酪氨酸磷酸化和蛋白质表达的影响。
Objective the study investigated the effect of gastrin on tyrosine phosphorylation and protein expression of focal adhesion kinase (FAK) in human colon cancer cells.
采用免疫沉淀及增强化学发光法检测IRS 1蛋白质酪氨酸磷酸化;
The tyrosine phosphorylation of IRS-1 was measured by immunoprecipitation and enhanced chemiluminescent immunoblotting technique.
鉴定了分子量为130000酪氨酸磷酸化的蛋白质为JAK2,一种非受体型酪氨酸激酶。
The tyrosine phosphorylated 130000 protein was identified as JAK2, a non receptor tyrosine kinase.
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