软琼脂克隆形成率随着照后传代次数的增加而明显增高；
The colony forming efficincy in soft agar increased with the passaging.
结果显示永生化胚胎肝细胞克隆形成率为31.2%，染色体核型分析表明细胞核型无明显异常，软琼脂集落形成试验表明细胞在软琼脂中不能生长。
It is showed that the cell clone-forming rat was 31. 2%. The immortalized human fetal hepatocytes had a normal karyotype and were not able to grow in soft AGAR culture.
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