目的：建立一种高效、快速的大肠杆菌感受态细胞制备及质粒转化的方法。
Objective: To establish an efficient and rapid method for the preparation and transformation of competent cells from Escherichia coli.
枯草芽孢杆菌感受态细胞的质粒转化与质粒的分子构型有关。
The transformability of plasmid DNA in B. subtilis competent cell depends on its molecular structure.
连接产物转化至BL21感受态细胞，涂板、挑选单克隆进行菌落PCR。
The products were transformed into BL21 competent cells, coated plates, select monoclonal colony PCR.
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