转染(transfection)是真核细胞在一定条件下主动或被动导入外源DNA片段而获得新的表型的过程。从本质上讲,和转化没有根本的区别。无论是转染还是转化,其关键因素都是用氯化钙处理大肠杆菌细胞,以提高细胞膜的通透性,从而使外源DNA分子能够容易进入细胞内部。所以在习惯上,人们往往也通称转染为广义的转化。常规转染技术可分为瞬时转染和稳定转染(永久转染)两大类。
转染 [遗] transfection ; Electransfection ; Dendritic Cell ; transfer
基因转染 gene transfer ; transfection ; gene transfer techniques
细胞转染 cell transfection ; Gene expression ; transfection ; The infected cells
电转染 electro transfer
转染试剂 Transfection reagents ; sirna ; Transfast ; FUGene HD Transfection Reagent
脂转染 lipofection
转染瘤 transfectoma
转染率 [遗] transfection efficiency
瞬时转染 Transient transfection
IL18基因转染 IL18 gene transfection
The transfection was identified with Northen blot hybridization.
结论:转染B。
参考来源 - 转染BclThe expression rate of E7 protein was 47.5%,which suggested efficient gene transfer.
E7蛋白的阳性表达率为47.5%,说明基因转染成功。
参考来源 - 负载HPV18E7基因脐带血树突状细胞的体外抗食管癌研究Adenovirus vectors have high efficency in gene transduction in adult islet cells and rat islet cells.
3、腺病毒载体对成人胰岛及大鼠胰岛具有较高的转染效率,在胰岛细胞内表达时间较长。
参考来源 - 腺病毒载体转染HO·2,447,543篇论文数据,部分数据来源于NoteExpress
最近的研究表明脂质体在转染呼吸道上皮细胞中是有用的。
Recent studies have shown lipoplexes to be useful in transfecting respiratory epithelial cells.
这一结果在新霉素包含子转染中也得到了证实。
This result was also confirmed by neomycin gene-containing replicons with same mutations.
令人担心的是它会转染到那些很容易在病人间传播的细菌中。
The fear would be that it gets into a strain of bacteria that is very good at being transmitted between patients.
应用推荐