报道了含sv40复制起点的质粒dna在真核细胞抽提物中进行复制的DNA体外复制系统的建立。

In vitro DNA replication system derived from eukaryotes is capable of replicating exogenous plasmid DNA containing SV40 origin of replication efficiently and accurately.

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该质粒可用于筛选水稻等真核生物DNA中的自主复制序列。

It's very useful for the cloning and expression of the autonomously repli-acting sequence from the eukaryotic DNA such as rice DNA.

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本发明还描述含有该基因的质粒，其在肠杆菌科细菌中可自主复制。

A plasmid containing the gene which is autonomously replicable in Enterobacteriaceae bacteria is also described.

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It has to have an origin of replication, which we talked about last time, which is compatible with the cells.

所以质粒必须带有复制起点,我们上次讲过,该起点要与受体细胞匹配

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So what you get on the plate is many copies of the small number of plasmids that you've put in.

我们只要放入少量质粒,就能在培养基上得到大量的复制品

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Now the plasmid started to do its thing, which is replicate and the gene gets transcribed.

那么质粒就可以开始进行复制,基因得以转录

耶鲁公开课 - 生物医学工程探索课程节选