研究病菌角质酶和胞壁降解酶种类及其在致病中的作用,可以为进一步阐述病菌的致病机理提供理论依据。
Study the cell-wall degrading enzymes (CWDEs) and cutinase by Botrytis cinerea and their pathogenicity to plant could make us best understanding about pathogenic mechanisms of plant pathogens.
方法:采用分离酶和胰蛋白酶联合消化法分离小型香猪角质形成细胞,角质形成细胞培养基培养。
METHODS: Keratinocytes of mini fragrant pigs were separated by dispase and trypsin combined digestive method, and then cultured with keratinocyte culture medium.
马拉色菌能像酯酶一样分解脂质,而且可引起角质形成细胞形态学改变和细胞凋亡,这些都是它的主要致病因素。
Malassezia can breakdown lipid like esterase, and result in morphological changes of malpighian cell and cell apoptosis, these are all their main etiological factors.
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