...究_好大夫在线 关键词】 角膜缘上皮细胞， 低温保存， 细胞培养， PCNA [gap=1531]Key wordsl Corneal limbal epithelial cells； Cryopreservation； Cell culture； PNCA ...
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以去除上皮细胞的羊膜为载体,采用三种方法对兔角膜缘上皮细胞(limbal epithelial cell ,LEC)进行接种培养,分别为:组织块直接接种培养法、组织块制备单细胞培养法、组织块经酶消化处理后培养法。
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以去除上皮细胞的羊膜为载体,采用三种方法对兔角膜缘上皮细胞(limbal epithelial cell ,LEC)进行接种培养,分别为:组织块直接接种培养法、组织块制备单细胞培养法、组织块经酶消化处理后培养法。
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探讨人角膜缘上皮细胞 corneal limbal epithelial cells
鸡角膜缘上皮细胞 cultured chicken limbal epithelial cell
角膜缘上皮干细胞 LESC
结论兔角膜缘上皮细胞可以在体外培养、冻存和复苏。
Conclusion the limbal corneal epithelial cells of rabbit could be cultivated, cryopreserved and resuscitated in vitro.
目的建立兔角膜缘上皮细胞体外培养、冻存和复苏的方法。
Objective to establish a method of cultivating limbal corneal epithelial cells of rabbit in vitro and to study its cryopreservation and resuscitation.
目的：建立体外培养兔角膜缘上皮细胞的方法，观察其体外生长的生物学特性。
Objective:To establish a method for culturing limbal corneal epithelial cells of rabbit and to observe the growth characteristics of the cultured cells in vitro.
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