建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
用吸附后的血清通过菌落原位免疫杂交筛选该基因组表达文库，从3000个菌落中获得了12个阳性克隆。
The genomic expression library was probed used the adsorbed-pooled sera, and 12 positive clones were obtained from 3000 postulating colonies by immunological hybridization.
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