The former showed similar results to parthenogenesis pathway, while the latter led to higher transformation and expression efficiency and 3.5% zygotic sporophytes were stained all-blue, implying the integration of lacZ at one-cell stage.
其中,雌配子体途径(转化后与正常雄配子体受精)的结果与孤雌生殖途径相仿,而雄配子体途径(转化后与正常雌配子体完成受精)可大幅提高转化效率与表达效率,有3.5%的植株实现均一性表达,提示外源基因的整合发生在单细胞阶段。
参考来源 - 海带遗传转化模型的优化及目的基因的导入·2,447,543篇论文数据,部分数据来源于NoteExpress
同一载体在不同种属细胞内表达效率不同。
Expression efficiency of the same expression vector is different in cells from different species.
目的:优化哺乳动物细胞表达系统,提高目的基因的表达效率。
Objective: to increase the expression level of foreign gene by the optimization of the expressional regulatory elements in the cassette of interesting gene.
目的探讨胚胎大鼠神经干细胞的体外培养条件及外源基因的表达效率。
Objective to explore the culture conditions for the neural stem cells from embryonic rat cortices and to test their efficacy of expressing exogenous gene.
That's our expression for the efficiency.
这就是效率的表达式。
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