蛋白质的沉淀(protein precipitation),沉淀是溶液中的溶质由液相变成固相析出的过程。蛋白质从溶液中析出的现象,称为蛋白质的沉淀。蛋白质沉淀常用的方法有盐析、等电点沉淀、有机溶剂沉淀、生物碱试剂与某些酸(如三氯醋酸)沉淀等。
用免疫共沉淀的方法鉴定 E4B 的相互作用蛋白质。
Co-immunoprecipitation was used to identify the interactive protein.
如果溶液远离这个等电位点,那么蛋白质会是高度可溶的因此表面张力梯度就无法形成;如果太接近这个等电位点,蛋白质会析出沉淀。
If the solution is too far from the IEP, the proteins are highly soluble and surface tension gradients never develop; too close, and the proteins precipitate out.
通过沉淀将病毒物质浓缩。由于病毒是蛋白质性质的,它们能通过蛋白质沉淀法沉淀。
Concentration of the virus particles by precipitation Because viruses are proteinaceous, they can be precipitated by methods that will precipitate proteins.
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