方法用邻苯三酚红法测定尿蛋白的重复性、回收率、线性范围。
Methods The repetition, recovery ratio and linearity of urinary protein were determined with pyrogallol red.
建立了TSF蛋白的纯化复性方法，获得了GST - TSF融合蛋白及TSF蛋白。
The purification procedure was established, and the purified TSF and its fused protein GST TSF were obtained.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
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