...PCR和RACE技术克隆藏羚羊HIF-1α基因的cDNA序列，同时采用real-time PCR和western blot方法检测藏羚羊、藏系绵羊(Tibetan sheep)、平原绵羊(Ovis aries)HIF-1α的组织特异性表达并进行比较分析。
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The following results were obtained in this study:1. The complete CDS of VEGF(573bp), partial sequence of IGF-I(940bp) and HGF(108bp) of Tibetan sheep were cloned successfully.
本研究取得了如下结果:1.成功克隆出了藏系绵羊VEGF基因全长CDS共573bp、IGF-ⅠcDNA序列共940bp和HGF基因cDNA的部分序列,长度为108bp。
参考来源 - 藏系绵羊三种促毛生长因子基因的克隆及营养差异对其mRNA表达的影响·2,447,543篇论文数据，部分数据来源于NoteExpress
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