方法：免疫荧光组织化学双重标记技术。
METHODS: Immunofluorescence histochemical double-staining method was used.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
方法：应用荧光组织化学方法对青春期大鼠髁突软骨中的ER进行检测分析。
Methods: Luminescent histochemical method was used to detect the ER in the condylar cartilage in young growing SD rats.
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