荧光物质当被短光波的紫外线放射时,则发出可见光线。荧光显微术即应用这一原理。荧光显微镜以紫外光线为光源,观察的标本多用荧光色素染色,由于紫外线的照射,激发标本内的荧光物质,而呈现荧光映像。
第三章中我们以第二章所提出的细胞内荧光显微术,结合落射荧光显微术 (Epi.FM)和激光扫描共聚焦荧光显微术(LSCFM),对活的人正常肝细胞 HL-7702中胰岛素受信号转导早期阶段在单分子水平进行了研究。
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全内反射荧光显微术 TIRFM ; total internal reflection fluorescence microscopy
免疫荧光显微术 immunofluorescence microscopy
直接荧光显微术 direct fluorescence microscopy
落射荧光显微术 Epi-fluorescence spectroscopy
显微荧光光度术 microfluorometry ; [光] [生物] microfluorophotometry
荧光显微镜技术 fluorescence microscopy
荧光显微技术 Fluorescence Microscopy
荧光显微镜检术 fluorescence microscopy
免疫荧光显微镜术 immunofluorescence microscopy
这是首次用全内反射荧光显微术测定了细胞中的内吞转铁蛋白形成内涵体的过程。
Formation of single endosomes on cell membrane was analysised by total internal reflection fluorescence microscopy at first.
利用荧光显微术观察,发现同源四倍体水稻与其二倍体原种的糊粉层细胞壁纤维素物质都存在一个“充实”的过程。
The formation of the aleuronic layer cell wall in autotetraploid and its original diploid rice was studied by using the technique of fluorescence microscopy.
该方法应用荧光显微术使单个DNA分子成像,这些单个的DNA分子已经被分成有序的片断,因而被称为限制性内切酶的识别序列。
The method USES fluorescence microscopy to image individual DNA molecules that have been divided into orderly fragments by so-called restriction enzymes.
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