目的从大鼠的早期胚胎分离和培养胚胎干细胞。

Objective To isolate and culture mouse embryonic stem cell from embryos of mouse.

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方法取大鼠胚胎分离成纤维细胞，分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。

Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.

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从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。

PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.

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Now I isolated these cells maybe from the head region of the embryo, the region that's going to develop into the brain, so that would be a good place to look if you wanted cells that were going to develop into the brain.

现在我可以从胚胎的头部,分离这些细胞,从就要发育成为大脑的这些部位,这是取得你需要的细胞的绝佳位置,如果你想要发育成大脑的细胞的话

耶鲁公开课 - 生物医学工程探索课程节选