采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
用聚合酶链反应和单链构象多态性（ PCR-S SCP）结合序列测定方法，检测了66例健康对照I L-1 0启动子区多态性的发生。
The Polymorphisms of IL 10 Promoter region were detected by PCR SSCP and sequencing. 66 of health control were examined by this way.
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