巢蛋白在P19神经元分化过程中的表达* =1∶2∶1)-20℃处理30min。 细胞经梯度甲醇换至PBS中, 在含有1% BSA、 0.5%正常羊血清(normal goat serum, NGS)的PBS封闭液中室温下静置30min, 然后在室温下与一抗作用1.5h, 兔抗巢蛋白抗体按1∶200
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接著 加入0.3% H 2 O 2 浸泡,可去除内生性的过氧化氢酶,处理30 分钟后, 以0.1M PBS 洗3 次,再加入10% 羊血清 (goat serum),处理30 分 钟,可降低染色之背景,之后加入所需的初级抗体,本实验以 anti-neuron specific enolase (anti-NSE) 鉴别神经细胞、...
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采用聚丙烯酰胺凝胶电泳法对100只欧拉羊血清淀粉酶同工酶的多态性特征进行了研究。
Oula sheep were used to investigate the polymorphism character of isozyme of serum amylase by means of polyacrylamide gel electrophoresis.
超纯正常羊血清在发光和荧光免疫组化和免疫细胞化学方法中作为封闭液和抗体稀释液使用。
Premium quality normal goat serum for use as blocking reagent and antibody diluent for chromogenic and fluorescent immunohistochemical and immunocytochemical assays.
结果:自制兔抗羊免疫血清和羊血清经环状沉淀、琼脂扩散多种沉淀反应试验,验证均与兔抗人血清和人血清的反应结果相近。
Because it has shown a pleased result in many kinds of precipitation reaction such as ring precipitation reaction, AGAR diffusion reaction and so on.
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