方法:采用全细胞膜片钳技术,记录钠通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法传代得到单个HERG-HEK细胞,应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线(激活、失活、复活和去活化)。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法全细胞膜片箝技术。
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