1.2 细胞群体倍增时间(population doubling time,PDT):指数生长期的细胞经质量浓度为2.5g/L胰蛋白酶消化成单细胞后,1×105个细胞接种于直径6cm培...
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与阴性对照组比较,阳性对照组和实验组细胞群体倍增时间增加。
Compared with negative control group, the doubling time of both positive control group and experimental groups was prolonged.
细胞群体倍增时间由第7代起显著延长(P<0.01); MTT比色法的结果亦显示,第7代细胞的增殖能力开始大幅下降(P<0.01)。
Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).
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