(2)低温 下膜系极性(polarity)会发生改变,造成膜系伤害,而冷冻保护剂也 会改变细胞环境(cellular environment),亦造成膜系极性改变。
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细胞内环境稳态 cellular environmental homeostasis
提供信息的细胞的环境 DICE
细胞外环境 Extracellular environment
构成了脑细胞的微环境 BEM ; brain extracellular microenvironment
细胞因子环境 cytokine milieu
脑细胞的微环境 brain extracellular microenvironment
细胞因子的环境 cytokine milieu
Such in vivo methods arehampered by one or more of the following factors: the inability to select under conditionsdifferent from the cellular environment, problems with the selection of proteins that are toxic,cells circumventing selection pressure and low transformation efficiency.
体内法主要受以下一种或几种因素而影响筛选效率:①不能在不同的细胞环境下进行筛选;②不能对对细胞有毒性的蛋白进行筛选;③细胞循环筛选压力;④较低的转化效率。
参考来源 - 人肺癌单链抗体库的构建、筛选及其抗体结构与功能的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
他们发现这些胎儿干细胞在细胞环境中同样可以自发分化为心脏细胞。
They found that the cells spontaneously differentiated into cardiac cells in cell culture as well.
可以想到宿主细胞本身或受体细胞获取外泌体的能力取决于细胞环境和生理状态。
It is conceivable that the ability of up-taking exosomes by host cells themselves or by recipient cells depends on cellular context, and physiological status.
尚未清楚的是什么样合适的细胞环境,和什么样的细胞线索触发了这个再生过程和迁移到发生地。
What's not known is what the right cellular environment is, and what the cellular cues are for this process of regeneration and migration to take place.
Or it could be not having to do with the cells themselves but maybe the environment that the cell finds itself in.
这种不同也可能和细胞本身无关,而和细胞所处的环境的变化有关
In fact, fibroblasts, which I talked about before, are very good producers of collagen, they produce the collagen matrix within which they live.
实际上,我刚讲过的,成纤维细胞,尤善于此,它们制造了自己生活环境中的,胶原蛋白基质
It could be those differences that they experience in their extracellular environment that lead to their choice to either self-renew or to become committed.
有可能就是由于,这种细胞外环境的不同,最终导致它们自我更新,或者成为定向的祖细胞
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