最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。
Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.
PR的表达情况：PR阳性表达定位于细胞核内，为棕黄色染色。
The expression of PR: The positive expression of PR is pale brown in cell nucleus.
方法：应用SP免疫组化法，检测P 21, P 27等细胞周期依赖性激酶抑制剂和增殖细胞核抗原(pcna)在角膜上皮不同部位的表达情况。
METHODS: the expressions of CKI, P27, P21 and proliferating cell nuclear antigen (PCNA) were tested in different regions of corneal epithelium by SP immunohistochemistry.
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