Methods: manipulate them to single cell suspension, detect the content of CD44~+CD24~(-/low) cells by flow cytometry (FCM).
实验方法:将乳腺癌组织、淋巴结和血液标本分别制成细胞悬液,利用流式细胞术（FCM）检测CD44+CD24-/low细胞的含量。
参考来源 - CD44~+CD24~（·2,447,543篇论文数据，部分数据来源于NoteExpress
收集细胞悬液，再经低渗处理后滴片染色。
Cell suspension was collected, and then stained after hypotonic treatment.
所回收细胞悬液中活细胞量用染料排除试验超过90%。
The amount of viable cells present in the recovered cell suspension exceeds 90% as examined by dye exclusion test.
把软骨细胞悬液直接滴加到PGA支架上进行复合培养。
The chondrocytes were seeded on the PGA scaffolds directly in control group.
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