目的：克隆人和小鼠的活化t细胞表达与分泌调节基因(RANTES基因)并分别构建腺病毒表达载体。
Objective: to clone the human and murine origin RANTES genes and construct the adenoviral expression vectors.
结论构建了人瘦素的哺乳动物细胞表达载体，并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
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