细胞克隆技术泛指所有体外培养,其含义是指从动物活体体内取出组织,于模拟体内生理环境等特定的体内条件下,进行孵育培养,使之生存并生长。细胞培养工作现已广泛应用于生物学、医学、新药研发等各个领域,成为最重要的基础科学之一。
方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞;
Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
相关学者从不同方面进行了研究,试图提高体细胞克隆技术的成功率。
Many researches were carried out in order to improve the efficiency of this technique.
综述了近年来哺乳动物体细胞克隆技术的研究进展,为其相关研究提供必要的参考。
In this article, the research progress on mammal somatic cell cloning related theories was reviewed, thus can be able to offer some refers for this technology research.
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