应用MTT法测定脾淋巴细胞转化指数。用免疫荧光法测定脾t淋巴细胞亚群细胞数，观察其对免疫功能的影响。
Spleen lymphocyte transforming index was measured with MTT method, and subtypes of t lymphocyte were counted by immunofluorescence to observe the influence on the immunological function.
方法采用APAAP桥联酶标法测定外周血t细胞亚群。
Methods t lymphocyte subpopulation in peripheral blood was measured by APAAP method.
目的测定围术期外周血T淋巴细胞亚群的变化，旨在探讨术中及术后连续硬膜外镇痛对免疫功能的影响。
Objective To investigate the influences of continuous epidural analgesia on immune function, authors observed the changes of peripheral blood T lymphocyte subsets during perioperative period.
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