The main results:(1) Primary murine lung fibroblasts were purified with high purity by lung tissue method and different timely adhensive method.
主要结果:1.应用肺组织块法及差时贴壁法相结合原代培养小鼠肺成纤维细胞,得到高纯度肺成纤维细胞。
参考来源 - NFMETHODS: VX2 tumor fragments were isolated and cultured with explant culture technique and enzymatic digestion in vitro. After 40 passages, the cell morphology, cell cycle, karyotype and tumorigenecity in rabbits and nude mice were investigated.
方法 采用组织块法和消化法结合对兔VX2肿瘤进行原代培养，体外传代观察，传代40次并对培养细胞进行形态学观察、细胞周期检查、核型分析、兔及裸鼠移植。
参考来源 - 热疗对兔VX2肿瘤细胞转移的影响Methods Normal dental pulp of teenagers were collected and human pulp fibroblast was cultured using tissue explant method.
方法选用年轻健康的人牙髓组织,以组织块法体外原代培养人牙髓成纤维细胞。
参考来源 - 自酸蚀粘结系统与牙髓组织生物相容性的实验研究·2,447,543篇论文数据，部分数据来源于NoteExpress
方法用组织块法培养人瘢痕成纤维细胞。
Methods The hypertrophic scar fibroblasts were cultured with tissue culture method.
改良组织块法是一种良好的大鼠牙囊细胞培养方法。
Improved explant culture was a good culturing method for rat DF cells.
资料综合：许旺细胞的培养方法包括组织块法和酶消化培养法。
DATA SYNTHESIS: The Schwann cells could be cultured by tissue block method and enzymes digesting method.
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