组成型表达的定义是基因表达不受时期、部位、环境影响,没有时空特异性。
正常状态下,某些HSP在细胞中具有一定量的组成型表达(constitutive expression),即基础表达,如HSP90β、HSC70、GRP78等。
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So far as I know, the majority of plant disease resistance genes are constitutive expression; even though also reported for induced gene, such as xal, which has been the only induced gene reported in the paper.
就现在所知而言,大多数植物抗病基因为组成型表达;尽管也有诱导表达的,如Xal,但是目前知道的诱导表达的基因仅这一个。
参考来源 - 花生各组织混合全长cDNA文库的构建和RGA片段的克隆Error prone PCR and chemical mutation are used to generate gene diversity and a constitutive expression vector pBS-ks(sv)is used to creat the mutants library.
本文采用易错PCR和化学诱变来建立分子的多样性,以组成型表达载体pBS-ks(sv)作为载体建立突变体文库。
参考来源 - 新型β·2,447,543篇论文数据,部分数据来源于NoteExpress
结论:为下一步在毕赤酵母中组成型表达外源蛋白,研究其作用机理和遗传机制奠定了基础。
Conclusion:The GAP promoter can be used to express foreign protein effectively and is the basis for studying the physicochemical properties and genetic mechanism of Gassericin T.
不同材料间具有稳定表达的组成型RRM RNA结合蛋白基因,同时具有丰富的差异表达的具调控功能的RRM RNA结合蛋白基因。
The primer was designed based on consensus sequence from RRM RNA binding protein gene family. The results showed that there were stable expressed fragments among the different mRNA samples.
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