方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法采用基因克隆、测序及生物信息学技术，分析HLA新等位基因与HLA已知基因序列的差异。
Methods Molecular cloning, sequencing and bioinformatics techniques were used to identify the difference between the new HLA allele and other known alleles.
方法利用聚合酶链反应-序列特异引物(PCR - ssp)法，对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
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