稳定转染或持久的转染是用于建立克隆的细胞系,这种细胞系中转染的目的基因整合到染色体DNA中并指导适量目的蛋白的合成。
After stable transfection, the mRNA and protein level of TCF4 gene were up-regulated in dermal papilla cells and the proliferation of dermal papilla cells was promoted.
经过稳定转染在毛乳头细胞中上调了TCF4基因mRNA和蛋白水平表达,促进了毛乳头细胞的增殖和外分泌功能。
参考来源 - TCF4/pcDNA3.0表达载体的构建及其在毛乳头细胞中的表达Fifthly, during the early stage of transient transfection and stable transfection periods, the expression level of TPO eucaryon expressed vectors piloted by P1A3 promoter is markedly higher than that of TPO eucaryon expressed vectors piloted by BLG3.9 and BLG1.9 promoters.
第五,在瞬时转染及稳定转染早期,P1A3启动子调控的TPO真核表达载体的表达水平要明显高于BLG3.9和BLG1.9启动子调控的TPO真核表达载体。
参考来源 - 乳腺特异表达人血小板生成素基因载体的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
逆转录病毒;稳定转染;干细胞。
Retrovirus vector; Stable transfected cell line; Stem cells.
大鼠骨髓间质干细胞可以成为PEX基因稳定转染的载体细胞吗?
Can bone marrow mesenchymal stem cells be used as PEX gene stable transfection vectors?
目的:在中国仓鼠卵巢(CHO)细胞上稳定转染咪唑啉1受体。
Objective: to stably transfect imidazoline-1 receptor (I 1r) in Chinese hamster ovary (CHO) cells.
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