稳定表达（stable expression）： 外源基因整合到了染色体上，可稳定地表达 并遗传下去。要获得转基因植物或动物，就必须 达到稳定表达。
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稳定表达报告蛋白 AKAR
稳定表达细胞系建立 Package
CHO稳定表达细胞株 CHO stable expression cell strain
的稳定表达标签 Robust Gene Signature
表达稳定性 Expression stability
These results indicated that DLC-1 stable expression blocked 5-8F cells at G0/G1 phase resulting in attenuated proliferation and colony forming ability in vitro and lower tumorigenicity potential in vivo.
提示DLC-1基因稳定表达后,在体外,通过使5-8F细胞停滞于G0-G1期导致5-8F细胞的增殖能力和克隆形成能力降低,在体内,肿瘤细胞的成瘤能力下降。
参考来源 - 鼻咽癌中抑瘤基因DLCAfter introducing the recombinant plasmid into package cells,the cell culture supernatant was used to infect 3T3-L1 cells,which were then screened for stably expressing β klotho gene.
将该载体导入包装细胞,收集病毒上清液并感染3T3-L1细胞,筛选到稳定表达βklotho细胞系。
参考来源 - 筛选糖尿病候选药物FGFTherefore, the study aims to clone human and mouse CXCR3 gene and establish gene transfection cell lines L929-huCXCR3 and L929-mCXCR3 with stably expression of human/mouse CXCR3 molecule, further investigates migration of L929-huCXCR3 and L929-mCXCR3 under the effect by CXCR3.
因此,本研究旨在克隆人和小鼠CXCR3基因,建立稳定表达人/小鼠CXCR3分子的基因转染细胞株L929-huCXCR3和L929-mCXCR3,研究L929-huCXCR3和L929-mCXCR3在CXCR3配体作用下的迁移能力。
参考来源 - 人及小鼠CXCR3基因转染细胞株的构建与鼠抗人CXCR3单克隆抗体的研制及生物学功能研究The results of stable expression showed that HbCBL2 was located at tonoplast and HbCBL1 was located at plasma memebrane,additionally,both HbCBL2 and HbCBL1 are located at the guard cells.
稳定表达结果表明,HbCBL2主要定位于液泡膜上;HbCBL1主要定位于质膜和细胞核;同时这两个蛋白在保卫细胞中也有表达。
参考来源 - 野大麦HbCBL1和HbCBL2结构与亚细胞定位分析Western blot analysis showed that Bbchitl gene expressed stably in transgenic tobacco.3.
Western blot分析也表明，Bbchit1基因在转基因烟草中能稳定表达目标蛋白。
参考来源 - 球孢白僵菌几丁质酶基因Bbchit1抗病作用研究·2,447,543篇论文数据，部分数据来源于NoteExpress
结果：建立了新基因HBRP稳定表达的基因工程细胞系。
Results: the stable expression cell system of the novel gene HBRP was constructed.
外源基因在大肠杆菌染色体上的稳定表达并不影响细菌的生长繁殖。
The stable expression of exogenous gene in E. coli chromosome had no effect on the bacterial growth and propagation.
GUS活性检测和胭脂碱分析表明，外源基因在转化细胞内得到稳定表达。
Enzyme activity of GUS and NOS assay demonstrated that foreign genes expressed in the transformed plants and calli.
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