经表型筛选和PCR鉴定证明目的片段已稳定整合到酵母染色体基因组中。
The positive clones, of which the chromosomes were integrated with the defensin gene, were identified by the phenotype and PCR.
摘要目的：建立稳定整合的细胞株，为研究外源基因定位整合机制及其对靶细胞生物特征影响，建立一个生物模型。
Objective: to establish a stable cell line and employ the cell line as a model to study target integration of exogenous cDNA and modulation of gene expression.
欧洲整合已经基本上把一个以好战出名的大陆转化为一个和平稳定的地区了。
European integration has already transformed most of a famously bellicose continent into a stable zone of peace.
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