关键词 DNA 甲基化 ； 甲基化检测方法 ； 芯片 [gap=7199]Keywords: DNA methylation ; methylation detection technology ; microarray
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方法：运用甲基化特异性P CR，检测RASSF1A基因在48例喉癌组织、相对应癌旁组织及48例正常人外周血中的启动子区甲基化情况。
Methods a methylation-specific PCR was performed to detect the promoter methylation of RASSF1A gene in 48 tumor tissues, 48 corresponding normal tissues and 48 normal blood plasma.
目的：建立检测肝癌胰岛素样生长因子ii (IGF - II)基因启动子p3甲基化状态的方法。
Aim: to detect the methylation at the promoter P3 of human insulin-like growth factor II (IGF-II) gene in hepatocellular carcinoma.
方法采用改良MS P法检测胃癌患者胃黏膜组织中DAPK基因的甲基化情况。
Methods Methylation state of DAPK in gastric cancer was detected by this improved MSP.
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